A cosa serve chemiluminescenza?

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A cosa serve chemiluminescenza?

A cosa serve chemiluminescenza?

La chemiluminescenza trova applicazione in chimica analitica per la determinazione qualitativa e quantitativa di svariate sostanze. Esempi applicativi sono la misura della concentrazione del monossido d'azoto (NO) nell'atmosfera e il saggio del luminolo, usato tra l'altro per il rilevamento di tracce di sangue.

Cosa è Elettrochemiluminescenza?

Un meccanismo nel quale può avere un ruolo di primo piano l'ECL, che si basa sulla generazione di un segnale luminoso misurabile a partire da uno stimolo elettrochimico. In questo caso, quindi, l'elettrochemiluminescenza permette di “accendere” gli anticorpi da trovare come fossero lampadine.

What is chemiluminescence and how does it work?

  • Chemiluminescence is the production of light from a chemical reaction. Two chemicals react to form an excited (high-energy) intermediate, which breaks down releasing some of its energy as photons of light (see glossary for all terms in bold) to reach its ground state (see Figure 1, below). A hydrogen atom in its ground state.

What is the chemical name for luminol?

  • Luminol (C 8 H 7 N 3 O 2) is a chemical that exhibits chemiluminescence, with a blue glow, when mixed with an appropriate oxidizing agent. Luminol is a white-to-pale-yellow crystalline solid that is soluble in most polar organic solvents, but less soluble in water.

What was the first chemiluminescent compound discovered?

  • The first chemiluminescent compound to be discovered was 2,4,5-triphenylimidazole (lophine), which was reported, in 1877, to emit light when mixed with potassium hydroxide in aqueous ethanol in the presence of air. A standard example of chemiluminescence in the laboratory setting is the luminol test.

What is the final step in infrared chemiluminiscence?

  • Final step: Subtract NO from (NO + NO 2) to yield NO 2 In chemical kinetics, infrared chemiluminiscence (IRCL) refers to the emission of infrared photons from vibrationally excited product molecules immediately after their formation.

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